Anxiety disorders and diabetes mellitus commonly occur simultaneously. Buspirone and diazepam are commonly administered drugs for the treatment of anxiety. When any drug is taken for long time it may have potential to produce alteration in metabolism. Present study is to see the effect of buspirone and diazepam on blood sugar levels in rabbits. The results show that these two drugs don’t affect the blood sugar level in rabbits and can be safely administered for the treatment of anxiety without alterations in the metabolic process.