Background: Malaria is associated with an increase in viral load and fall in CD4-cell count. This study was carried out to estimate the prevalence of malaria among HIV patients in hospitals. Method: This was a descriptive cross-sectional study that reviewed HIV patients’ folders in hospitals in Ghana. Ethical approvals were obtained from three recognized Ethical Review Committees. Result: The total 933 patients were made of 272 (29.2%) males and 661 (70.8%) females. A prevalence of 4.4% (95% CI: 2.2, 6.6) confirmed cases of malaria was recorded in the study. Fever, was not significantly associated with confirmed malaria (OR=3.11, 95% CI: [0.63, 15.37], P=0.142). Conclusions: A low prevalence of confirmed malaria, 4.4% (95% CI: 2.2, 6.6), was recorded among HIV patients from Ghana. The prevalence could also be attributed to the high cases of malaria diagnosed presumptively (37.0%), (OR=4.11, 95% CI: [2.83, 5.96], P...