"The interaction of Human Serum Albumin with Thymoquinone plays an important role in the pharmacology and pharmacodynamics in order to help us better understand the absorption and distribution of Thymoquinone in the body. This study showed that Human Serum Albumin as a model protein for elucidating Human Serum Albumin with Thymoquinone complex at different temperatures. Also we estimated the conformational stability, of Human Serum Albmin and its complex with Thymoquinonr against the urea action".